Recombinant:Article Title: Trophoblastic signals facilitate endometrial interferon response and lipid metabolism, ensuring normal decidualization.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Cytokeratin 8 (CK8) Millipore Cat# MABT329; RRID:AB_2891089 Anti-Placental lactogen I (PL-1) Santa Cruz Biotechnology Cat# sc-34713; RRID:AB_654194 Anti-LPL (LPL) GeneTex Cat# GTX101125; RRID:AB_1950830 Anti-Claudin 5 (CLDN5) Thermo Fisher Scientific Cat# 35–2500; RRID:AB_2533200 Anti-Stat1 (STAT1) Cell Signaling Technology Cat# 9172; RRID:AB_2198300 Anti-Phospho-Stat1 (p-STAT1) Cell Signaling Technology Cat# 9167; RRID:AB_561284 Anti-b-Tubulin ABclonal Cat# AC008; RRID:AB_2773006 Anti-Dtprp (PRL8A2) Homemade64 N/A Chemicals, peptides, and recombinant proteins Affi-Gel Blue Gel Beads Bio-rad Cat# 1537302 Recombinant mouse IL-6 MedChemExpress Cat# HY-P7378 Recombinant mouse IFN-Kappa R&D Systems Cat# 8437-MK Recombinant mouse Lipoprotein lipase CUSABIO Cat# CSB-MP013065MO BSA Sigma-Aldrich Cat# A9418 Orlistat (Tetrahydrolipstatin) MedchemExpress Cat# HY-B0218 NBT/BCIP Sangon Biotech Cat# C520019 INT/BCIP Sangon Biotech Cat# C500033 SplintR ligase NEB Cat# M0375L phi29 DNA Polymerase NEB Cat# M0269L CY3-Tyramide Servicebio Cat# G1223 TRIzol Invitrogen Cat# 15596018 BODIPY 493/503 GlpBio Cat# GC42959 4, 6-diamidino 2-phenyindiol Beyotime Cat# C1002 Critical commercial assays PrimeScript RT reagent Kit TAKARA Cat# RR047A TB Green Permix Ex Taq II TAKARA Cat# RR820A Prostaglandin (PG) E2 ELISA kit (Mouse) Solarbio Cat# SEKM-0173 Triglyceride content determination kit Solarbio Cat# BC0620 lipoprotein lipase assay kit Solarbio Cat# BC2445 Deposited data Raw and analyzed bulk RNA-seq data This paper GEO: GSE240086 TE and pTGC bulk RNA-seq data Niraimathi et al.34 GEO: GSE151329 Experimental models: Organisms/strains Pl1cre (Prl3d1-Cre) mouse line Ouseph et al.27 N/A Rosa26-DTA (R26-DTA) mouse line The Jackson Laboratory RRID:IMSR_JAX:032087 Rosa26-mTmG (R26-mTmG) mouse line The Jackson Laboratory RRID:IMSR_JAX:007576 Oligonucleotides Primers for in situ hybridization probes (Table S1) This paper N/A Primers for SCRINSHOT probes (Table S1) This paper N/A Primers for qRT-PCR analysis (Table S1) This paper N/A Software and algorithms R (v4.3.2) The R Development Core Team https://www.r-project.org Rstudio (v2023.09.1 + 494) RStudio Team http://www.rstudio.com (Continued on next page) 16 Cell Reports 43, 114246, June 25, 2024 ..
Enzyme-linked Immunosorbent Assay:Article Title: Trophoblastic signals facilitate endometrial interferon response and lipid metabolism, ensuring normal decidualization.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Cytokeratin 8 (CK8) Millipore Cat# MABT329; RRID:AB_2891089 Anti-Placental lactogen I (PL-1) Santa Cruz Biotechnology Cat# sc-34713; RRID:AB_654194 Anti-LPL (LPL) GeneTex Cat# GTX101125; RRID:AB_1950830 Anti-Claudin 5 (CLDN5) Thermo Fisher Scientific Cat# 35–2500; RRID:AB_2533200 Anti-Stat1 (STAT1) Cell Signaling Technology Cat# 9172; RRID:AB_2198300 Anti-Phospho-Stat1 (p-STAT1) Cell Signaling Technology Cat# 9167; RRID:AB_561284 Anti-b-Tubulin ABclonal Cat# AC008; RRID:AB_2773006 Anti-Dtprp (PRL8A2) Homemade64 N/A Chemicals, peptides, and recombinant proteins Affi-Gel Blue Gel Beads Bio-rad Cat# 1537302 Recombinant mouse IL-6 MedChemExpress Cat# HY-P7378 Recombinant mouse IFN-Kappa R&D Systems Cat# 8437-MK Recombinant mouse Lipoprotein lipase CUSABIO Cat# CSB-MP013065MO BSA Sigma-Aldrich Cat# A9418 Orlistat (Tetrahydrolipstatin) MedchemExpress Cat# HY-B0218 NBT/BCIP Sangon Biotech Cat# C520019 INT/BCIP Sangon Biotech Cat# C500033 SplintR ligase NEB Cat# M0375L phi29 DNA Polymerase NEB Cat# M0269L CY3-Tyramide Servicebio Cat# G1223 TRIzol Invitrogen Cat# 15596018 BODIPY 493/503 GlpBio Cat# GC42959 4, 6-diamidino 2-phenyindiol Beyotime Cat# C1002 Critical commercial assays PrimeScript RT reagent Kit TAKARA Cat# RR047A TB Green Permix Ex Taq II TAKARA Cat# RR820A Prostaglandin (PG) E2 ELISA kit (Mouse) Solarbio Cat# SEKM-0173 Triglyceride content determination kit Solarbio Cat# BC0620 lipoprotein lipase assay kit Solarbio Cat# BC2445 Deposited data Raw and analyzed bulk RNA-seq data This paper GEO: GSE240086 TE and pTGC bulk RNA-seq data Niraimathi et al.34 GEO: GSE151329 Experimental models: Organisms/strains Pl1cre (Prl3d1-Cre) mouse line Ouseph et al.27 N/A Rosa26-DTA (R26-DTA) mouse line The Jackson Laboratory RRID:IMSR_JAX:032087 Rosa26-mTmG (R26-mTmG) mouse line The Jackson Laboratory RRID:IMSR_JAX:007576 Oligonucleotides Primers for in situ hybridization probes (Table S1) This paper N/A Primers for SCRINSHOT probes (Table S1) This paper N/A Primers for qRT-PCR analysis (Table S1) This paper N/A Software and algorithms R (v4.3.2) The R Development Core Team https://www.r-project.org Rstudio (v2023.09.1 + 494) RStudio Team http://www.rstudio.com (Continued on next page) 16 Cell Reports 43, 114246, June 25, 2024 ..
RNA Sequencing:Article Title: Trophoblastic signals facilitate endometrial interferon response and lipid metabolism, ensuring normal decidualization.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Cytokeratin 8 (CK8) Millipore Cat# MABT329; RRID:AB_2891089 Anti-Placental lactogen I (PL-1) Santa Cruz Biotechnology Cat# sc-34713; RRID:AB_654194 Anti-LPL (LPL) GeneTex Cat# GTX101125; RRID:AB_1950830 Anti-Claudin 5 (CLDN5) Thermo Fisher Scientific Cat# 35–2500; RRID:AB_2533200 Anti-Stat1 (STAT1) Cell Signaling Technology Cat# 9172; RRID:AB_2198300 Anti-Phospho-Stat1 (p-STAT1) Cell Signaling Technology Cat# 9167; RRID:AB_561284 Anti-b-Tubulin ABclonal Cat# AC008; RRID:AB_2773006 Anti-Dtprp (PRL8A2) Homemade64 N/A Chemicals, peptides, and recombinant proteins Affi-Gel Blue Gel Beads Bio-rad Cat# 1537302 Recombinant mouse IL-6 MedChemExpress Cat# HY-P7378 Recombinant mouse IFN-Kappa R&D Systems Cat# 8437-MK Recombinant mouse Lipoprotein lipase CUSABIO Cat# CSB-MP013065MO BSA Sigma-Aldrich Cat# A9418 Orlistat (Tetrahydrolipstatin) MedchemExpress Cat# HY-B0218 NBT/BCIP Sangon Biotech Cat# C520019 INT/BCIP Sangon Biotech Cat# C500033 SplintR ligase NEB Cat# M0375L phi29 DNA Polymerase NEB Cat# M0269L CY3-Tyramide Servicebio Cat# G1223 TRIzol Invitrogen Cat# 15596018 BODIPY 493/503 GlpBio Cat# GC42959 4, 6-diamidino 2-phenyindiol Beyotime Cat# C1002 Critical commercial assays PrimeScript RT reagent Kit TAKARA Cat# RR047A TB Green Permix Ex Taq II TAKARA Cat# RR820A Prostaglandin (PG) E2 ELISA kit (Mouse) Solarbio Cat# SEKM-0173 Triglyceride content determination kit Solarbio Cat# BC0620 lipoprotein lipase assay kit Solarbio Cat# BC2445 Deposited data Raw and analyzed bulk RNA-seq data This paper GEO: GSE240086 TE and pTGC bulk RNA-seq data Niraimathi et al.34 GEO: GSE151329 Experimental models: Organisms/strains Pl1cre (Prl3d1-Cre) mouse line Ouseph et al.27 N/A Rosa26-DTA (R26-DTA) mouse line The Jackson Laboratory RRID:IMSR_JAX:032087 Rosa26-mTmG (R26-mTmG) mouse line The Jackson Laboratory RRID:IMSR_JAX:007576 Oligonucleotides Primers for in situ hybridization probes (Table S1) This paper N/A Primers for SCRINSHOT probes (Table S1) This paper N/A Primers for qRT-PCR analysis (Table S1) This paper N/A Software and algorithms R (v4.3.2) The R Development Core Team https://www.r-project.org Rstudio (v2023.09.1 + 494) RStudio Team http://www.rstudio.com (Continued on next page) 16 Cell Reports 43, 114246, June 25, 2024 ..
In Situ Hybridization:Article Title: Trophoblastic signals facilitate endometrial interferon response and lipid metabolism, ensuring normal decidualization.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Cytokeratin 8 (CK8) Millipore Cat# MABT329; RRID:AB_2891089 Anti-Placental lactogen I (PL-1) Santa Cruz Biotechnology Cat# sc-34713; RRID:AB_654194 Anti-LPL (LPL) GeneTex Cat# GTX101125; RRID:AB_1950830 Anti-Claudin 5 (CLDN5) Thermo Fisher Scientific Cat# 35–2500; RRID:AB_2533200 Anti-Stat1 (STAT1) Cell Signaling Technology Cat# 9172; RRID:AB_2198300 Anti-Phospho-Stat1 (p-STAT1) Cell Signaling Technology Cat# 9167; RRID:AB_561284 Anti-b-Tubulin ABclonal Cat# AC008; RRID:AB_2773006 Anti-Dtprp (PRL8A2) Homemade64 N/A Chemicals, peptides, and recombinant proteins Affi-Gel Blue Gel Beads Bio-rad Cat# 1537302 Recombinant mouse IL-6 MedChemExpress Cat# HY-P7378 Recombinant mouse IFN-Kappa R&D Systems Cat# 8437-MK Recombinant mouse Lipoprotein lipase CUSABIO Cat# CSB-MP013065MO BSA Sigma-Aldrich Cat# A9418 Orlistat (Tetrahydrolipstatin) MedchemExpress Cat# HY-B0218 NBT/BCIP Sangon Biotech Cat# C520019 INT/BCIP Sangon Biotech Cat# C500033 SplintR ligase NEB Cat# M0375L phi29 DNA Polymerase NEB Cat# M0269L CY3-Tyramide Servicebio Cat# G1223 TRIzol Invitrogen Cat# 15596018 BODIPY 493/503 GlpBio Cat# GC42959 4, 6-diamidino 2-phenyindiol Beyotime Cat# C1002 Critical commercial assays PrimeScript RT reagent Kit TAKARA Cat# RR047A TB Green Permix Ex Taq II TAKARA Cat# RR820A Prostaglandin (PG) E2 ELISA kit (Mouse) Solarbio Cat# SEKM-0173 Triglyceride content determination kit Solarbio Cat# BC0620 lipoprotein lipase assay kit Solarbio Cat# BC2445 Deposited data Raw and analyzed bulk RNA-seq data This paper GEO: GSE240086 TE and pTGC bulk RNA-seq data Niraimathi et al.34 GEO: GSE151329 Experimental models: Organisms/strains Pl1cre (Prl3d1-Cre) mouse line Ouseph et al.27 N/A Rosa26-DTA (R26-DTA) mouse line The Jackson Laboratory RRID:IMSR_JAX:032087 Rosa26-mTmG (R26-mTmG) mouse line The Jackson Laboratory RRID:IMSR_JAX:007576 Oligonucleotides Primers for in situ hybridization probes (Table S1) This paper N/A Primers for SCRINSHOT probes (Table S1) This paper N/A Primers for qRT-PCR analysis (Table S1) This paper N/A Software and algorithms R (v4.3.2) The R Development Core Team https://www.r-project.org Rstudio (v2023.09.1 + 494) RStudio Team http://www.rstudio.com (Continued on next page) 16 Cell Reports 43, 114246, June 25, 2024 ..
Quantitative RT-PCR:Article Title: Trophoblastic signals facilitate endometrial interferon response and lipid metabolism, ensuring normal decidualization.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Cytokeratin 8 (CK8) Millipore Cat# MABT329; RRID:AB_2891089 Anti-Placental lactogen I (PL-1) Santa Cruz Biotechnology Cat# sc-34713; RRID:AB_654194 Anti-LPL (LPL) GeneTex Cat# GTX101125; RRID:AB_1950830 Anti-Claudin 5 (CLDN5) Thermo Fisher Scientific Cat# 35–2500; RRID:AB_2533200 Anti-Stat1 (STAT1) Cell Signaling Technology Cat# 9172; RRID:AB_2198300 Anti-Phospho-Stat1 (p-STAT1) Cell Signaling Technology Cat# 9167; RRID:AB_561284 Anti-b-Tubulin ABclonal Cat# AC008; RRID:AB_2773006 Anti-Dtprp (PRL8A2) Homemade64 N/A Chemicals, peptides, and recombinant proteins Affi-Gel Blue Gel Beads Bio-rad Cat# 1537302 Recombinant mouse IL-6 MedChemExpress Cat# HY-P7378 Recombinant mouse IFN-Kappa R&D Systems Cat# 8437-MK Recombinant mouse Lipoprotein lipase CUSABIO Cat# CSB-MP013065MO BSA Sigma-Aldrich Cat# A9418 Orlistat (Tetrahydrolipstatin) MedchemExpress Cat# HY-B0218 NBT/BCIP Sangon Biotech Cat# C520019 INT/BCIP Sangon Biotech Cat# C500033 SplintR ligase NEB Cat# M0375L phi29 DNA Polymerase NEB Cat# M0269L CY3-Tyramide Servicebio Cat# G1223 TRIzol Invitrogen Cat# 15596018 BODIPY 493/503 GlpBio Cat# GC42959 4, 6-diamidino 2-phenyindiol Beyotime Cat# C1002 Critical commercial assays PrimeScript RT reagent Kit TAKARA Cat# RR047A TB Green Permix Ex Taq II TAKARA Cat# RR820A Prostaglandin (PG) E2 ELISA kit (Mouse) Solarbio Cat# SEKM-0173 Triglyceride content determination kit Solarbio Cat# BC0620 lipoprotein lipase assay kit Solarbio Cat# BC2445 Deposited data Raw and analyzed bulk RNA-seq data This paper GEO: GSE240086 TE and pTGC bulk RNA-seq data Niraimathi et al.34 GEO: GSE151329 Experimental models: Organisms/strains Pl1cre (Prl3d1-Cre) mouse line Ouseph et al.27 N/A Rosa26-DTA (R26-DTA) mouse line The Jackson Laboratory RRID:IMSR_JAX:032087 Rosa26-mTmG (R26-mTmG) mouse line The Jackson Laboratory RRID:IMSR_JAX:007576 Oligonucleotides Primers for in situ hybridization probes (Table S1) This paper N/A Primers for SCRINSHOT probes (Table S1) This paper N/A Primers for qRT-PCR analysis (Table S1) This paper N/A Software and algorithms R (v4.3.2) The R Development Core Team https://www.r-project.org Rstudio (v2023.09.1 + 494) RStudio Team http://www.rstudio.com (Continued on next page) 16 Cell Reports 43, 114246, June 25, 2024 ..
Software:Article Title: Trophoblastic signals facilitate endometrial interferon response and lipid metabolism, ensuring normal decidualization.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Cytokeratin 8 (CK8) Millipore Cat# MABT329; RRID:AB_2891089 Anti-Placental lactogen I (PL-1) Santa Cruz Biotechnology Cat# sc-34713; RRID:AB_654194 Anti-LPL (LPL) GeneTex Cat# GTX101125; RRID:AB_1950830 Anti-Claudin 5 (CLDN5) Thermo Fisher Scientific Cat# 35–2500; RRID:AB_2533200 Anti-Stat1 (STAT1) Cell Signaling Technology Cat# 9172; RRID:AB_2198300 Anti-Phospho-Stat1 (p-STAT1) Cell Signaling Technology Cat# 9167; RRID:AB_561284 Anti-b-Tubulin ABclonal Cat# AC008; RRID:AB_2773006 Anti-Dtprp (PRL8A2) Homemade64 N/A Chemicals, peptides, and recombinant proteins Affi-Gel Blue Gel Beads Bio-rad Cat# 1537302 Recombinant mouse IL-6 MedChemExpress Cat# HY-P7378 Recombinant mouse IFN-Kappa R&D Systems Cat# 8437-MK Recombinant mouse Lipoprotein lipase CUSABIO Cat# CSB-MP013065MO BSA Sigma-Aldrich Cat# A9418 Orlistat (Tetrahydrolipstatin) MedchemExpress Cat# HY-B0218 NBT/BCIP Sangon Biotech Cat# C520019 INT/BCIP Sangon Biotech Cat# C500033 SplintR ligase NEB Cat# M0375L phi29 DNA Polymerase NEB Cat# M0269L CY3-Tyramide Servicebio Cat# G1223 TRIzol Invitrogen Cat# 15596018 BODIPY 493/503 GlpBio Cat# GC42959 4, 6-diamidino 2-phenyindiol Beyotime Cat# C1002 Critical commercial assays PrimeScript RT reagent Kit TAKARA Cat# RR047A TB Green Permix Ex Taq II TAKARA Cat# RR820A Prostaglandin (PG) E2 ELISA kit (Mouse) Solarbio Cat# SEKM-0173 Triglyceride content determination kit Solarbio Cat# BC0620 lipoprotein lipase assay kit Solarbio Cat# BC2445 Deposited data Raw and analyzed bulk RNA-seq data This paper GEO: GSE240086 TE and pTGC bulk RNA-seq data Niraimathi et al.34 GEO: GSE151329 Experimental models: Organisms/strains Pl1cre (Prl3d1-Cre) mouse line Ouseph et al.27 N/A Rosa26-DTA (R26-DTA) mouse line The Jackson Laboratory RRID:IMSR_JAX:032087 Rosa26-mTmG (R26-mTmG) mouse line The Jackson Laboratory RRID:IMSR_JAX:007576 Oligonucleotides Primers for in situ hybridization probes (Table S1) This paper N/A Primers for SCRINSHOT probes (Table S1) This paper N/A Primers for qRT-PCR analysis (Table S1) This paper N/A Software and algorithms R (v4.3.2) The R Development Core Team https://www.r-project.org Rstudio (v2023.09.1 + 494) RStudio Team http://www.rstudio.com (Continued on next page) 16 Cell Reports 43, 114246, June 25, 2024 ..
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